Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC50 is 5.225-9.256 ng/ml.
Alternative Names
CLDN18; UNQ778/PRO1572; Claudin-18
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
1-261aa
Target Protein Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Protein Length
Full Length of Isoform A2
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form
Liquid
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer
PBS, pH 7.4, 6% trehalose
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA
Please contact us to get it.
Description
This recombinant human claudin-18.2 (CLDN 18.2) is prepared in mammalian cells. Its target gene encodes the full human claudin-18.2 (CLDN 18.2), containing a 10xHis-tag at the C-terminus. The CLDN 18.2 protein has been validated to be active through a functional ELISA. Immobilized human CLDN 18.2 protein can bind to the recombinant anti-CLDN 18.2 monoclonal antibody. And the EC50 is 5.225-9.256 ng/ml. In the WB assay, this CLDN 18.2 protein was detected by the mouse anti-6xHis monoclonal antibody. Three bands appeared on the gel electrophoresis, respectively corresponding to monomer, homodimer, homotrimer of the CLDN 18.2.
CLDN 18.2, the isoform of CLDN 18, is a tight junction protein exclusively expressed in differentiated epithelial cells of gastric mucosa under normal physiological conditions. Studies have demonstrated that CLDN 18.2 was highly expressed in primary gastric malignancies and post-metastatic types of cancer, including carcinomas of the pancreas, esophagus, ovaries, and lungs. Therefore, CLDN 18.2 has become an ideal target for researchers to develop immunotherapies for solid tumors such as gastric cancer and pancreatic cancer.
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